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Nucleic acid methods are the techniques used to study nucleic acids: DNA and RNA.

• Purification

• Quantification

• Synthesis

• Kinetics

• Gene function

• Other

• See also

• References

• External links

• DNA extractioncite-ref-1[1]

• Phenol–chloroform extraction

• Minicolumn purification

• RNA extraction

• Boom method

• Synchronous coefficient of drag alteration (SCODA) DNA purification

• Abundance in weight: spectroscopic nucleic acid quantitation

• Absolute abundance in number: real-time polymerase chain reaction (quantitative PCR)

• High-throughput relative abundance: DNA microarray

• High-throughput absolute abundance: serial analysis of gene expression (SAGE)

• Size: gel electrophoresis

• De novo: oligonucleotide synthesis

• Amplification: polymerase chain reaction (PCR), loop-mediated isothermal amplification (LAMP), transcription-mediated amplification (TMA)

• Multi-parametric surface plasmon resonancecite-ref-2[2]cite-ref-3[3]

• Dual-polarization interferometrycite-ref-4[4]

• Quartz crystal microbalance with dissipation monitoring (QCM-D)cite-ref-5[5]

• RNA interferencecite-ref-6[6]

• Bisulfite sequencing

• DNA sequencing

• Expression cloning

• Fluorescence in situ hybridization

• Lab-on-a-chip

• Comparison of nucleic acid simulation software

• Northern blot

• Nuclear run-on assay

• Radioactivity in the life sciences

• Southern blot

• Differential centrifugation (sucrose gradient)

• Toeprinting assay

• Several bioinformatics methods, as seen in list of RNA structure prediction software

• CSH Protocols

• Current Protocols

cite-note-11. ↑ citerefrana2025Rana, Ajay K. (2025). "Challenging biological samples and strategies for DNA extraction". Journal of Investigative Medicine. 73 (6): 443–459. doi:10.1177/10815589251327503. PMID 40033560.

cite-note-22. ↑ citereftanghuwangzhao2014Tang, Wei; Hu, Shichao; Wang, Huaming; Zhao, Yan; Li, Na; Liu, Feng (23 September 2014). "A universal molecular translator for non-nucleic acid targets that enables dynamic DNA assemblies and logic operations". Chem. Commun. 50 (92): 14352–14355. doi:10.1039/C4CC07041K. PMID 25295484.

cite-note-33. ↑ citerefihalainenpetterssonpesonenviitala2014Ihalainen, Petri; Pettersson, Fredrik; Pesonen, Markus; Viitala, Tapani; Määttänen, Anni; Österbacka, Ronald; Peltonen, Jouko (7 March 2014). "An impedimetric study of DNA hybridization on paper-supported inkjet-printed gold electrodes". Nanotechnology. 25 (9): 094009. Bibcode:2014Nanot..25i4009I. doi:10.1088/0957-4484/25/9/094009. PMID 24522116. S2CID 206070778.

cite-note-44. ↑ citerefberneyoliver2005Berney, H.; Oliver, K. (2005). "Dual polarization interferometry size and density characterisation of DNA immobilisation and hybridisation". Biosensors and Bioelectronics. 21 (4): 618–626. doi:10.1016/j.bios.2004.12.024. PMID 16202875.

cite-note-55. ↑ citerefdixon2008Dixon, Matthew C. (July 2008). "Quartz Crystal Microbalance with Dissipation Monitoring: Enabling Real-Time Characterization of Biological Materials and Their Interactions". Journal of Biomolecular Techniques. 19 (3): 151–158. PMC 2563918. PMID 19137101.

cite-note-66. ↑ citerefhannon2002Hannon, Gregory J. (July 2002). "RNA interference". Nature. 418 (6894): 244–251. Bibcode:2002Natur.418..244H. doi:10.1038/418244a. ISSN 1476-4687. PMID 12110901. S2CID 4426281.

• Protocols for Recombinant DNA Isolation, Cloning, and Sequencing